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Thymosin Alpha-1 Calculator — Reconstitution & Dose

Concentration, draw volume & U-100 units for 1.6 mg, 5 mg and 10 mg vials

A free thymosin alpha-1 reconstitution calculator. Enter the vial size, the liquid you added and the dose you want to convert, and it works out the concentration, the draw volume and the U-100 syringe mark. It converts numbers you already have. It does not suggest a dose.

Enter your numbers

mg

Total amount in the vial, as printed on its label.

mL

How much water went into the vial. Ready-mixed vial? See the note below the calculator.

Dose unit

Enter the dose from your own records or prescription. This tool never suggests one.

Syringe size (U-100 insulin syringe)

Draw volume on a 1 mL U-100 syringe

Fill in the three fields and the math appears here instantly.

102030405060708090100
Concentration
Per unit mark
per U-100 unit
Draws per vial
at the dose entered

Arithmetic on the numbers you entered. Not a dose recommendation and not medical advice.

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How the Thymosin Alpha-1 calculator works

Lyophilized (freeze-dried) powder in a sealed vial, labelled in milligrams. The quick-fill buttons in the calculator are the vial sizes Thymosin Alpha-1 is commonly labelled in (1.6 mg, 5 mg and 10 mg). If yours is different, type it in.

Amounts of Thymosin Alpha-1 are usually written in milligrams (mg), so the calculator opens in mg.

If your vial is the licensed product, use the diluent and the volume given in its own instructions rather than any figure from this page.

For a field-by-field walkthrough and a guide to syringe types, see the main peptide calculator.

Thymosin Alpha-1 concentration table

Each labelled vial size at the same three round water volumes used across this site. It shows how the arithmetic behaves and is not a suggestion of what to use.

Thymosin Alpha-1 concentration by vial size and water volume
VialWater addedConcentrationEach mark holds
1.6 mg vial1 mL1.6 mg/mL0.16 mg per 10 units
1.6 mg vial2 mL0.8 mg/mL0.08 mg per 10 units
1.6 mg vial3 mL0.53 mg/mL0.053 mg per 10 units
5 mg vial1 mL5 mg/mL0.5 mg per 10 units
5 mg vial2 mL2.5 mg/mL0.25 mg per 10 units
5 mg vial3 mL1.67 mg/mL0.167 mg per 10 units
10 mg vial1 mL10 mg/mL1 mg per 10 units
10 mg vial2 mL5 mg/mL0.5 mg per 10 units
10 mg vial3 mL3.33 mg/mL0.333 mg per 10 units

Every row for a given vial holds the same total. To compare volumes for a specific reading, use the BAC water calculator.

The formula, step by step

Concentration (mg/mL) = vial size (mg) ÷ water added (mL)
Volume (mL) = dose (mg) ÷ concentration (mg/mL)
U-100 units = volume (mL) × 100

The “Try example numbers” button uses one fixed rule on every page: the smallest labelled vial, 2 mL of water, and whatever amount lands on the 10-unit mark. The amount is a by-product of that rule and not a typical or suggested Thymosin Alpha-1 dose.

  • Concentration: 1.6 mg ÷ 2 mL = 0.8 mg/mL.
  • The 10-unit mark is 10 ÷ 100 = 0.1 mL, which holds 0.1 × 0.8 = 0.08 mg.
  • Run it forwards to check: 0.08 mg ÷ 0.8 mg/mL = 0.1 mL, and 0.1 × 100 = 10 units.

Thymosin Alpha-1: general reference

General educational reference for research purposes only. None of it is a recommendation, none of it is medical advice, and none of it is used by the calculator, which works only on the numbers you enter.

Thymosin alpha-1 is a peptide of 28 amino acids that was first isolated from thymus tissue. The synthetic version is called thymalfasin.

Thymalfasin is sold as Zadaxin and is approved as a prescription medicine in more than 35 countries, including China and Italy. It has never been approved by the FDA.

The licensed product is a 1.6 mg vial of freeze-dried powder that comes with its own diluent and its own instructions.

Class
28-amino-acid thymic peptide
Group
Thymosins and BPC-157
Also known as
Thymalfasin, Zadaxin
Molecular weight
About 3,108 Da
Half-life
About 2 hours. Published reviews give a half-life in the circulation of about 2 hours.
Regulatory status
Approved in more than 35 countries, not by the FDA. Thymalfasin is an approved prescription medicine in more than 35 countries. It has never been approved by the FDA, and its position in US pharmacy compounding has changed since 2023, so check the FDA’s current lists for the present status.
Labelled vial sizes
1.6 mg, 5 mg and 10 mg

Arithmetic slips to watch for with Thymosin Alpha-1

The licensed vial is 1.6 mg

That is an unusual size, and the arithmetic follows it: 1.6 mg in 1 mL is 1.6 mg/mL, so 10 units on a U-100 syringe holds 0.16 mg. Research vials of 5 mg and 10 mg give very different concentrations with the same volume.

Not the same thing as TB-500

Thymosin alpha-1 and thymosin beta-4 (the parent of TB-500) share a name because both were first found in thymus extracts. They are unrelated molecules of different sizes. A number worked out for one tells you nothing about the other.

Watch the decimal point

1.6 and 16 differ by ten times. Amounts are written in milligrams with decimals, and the “Show the math” panel prints every step so a slipped decimal is easy to see.

What this page does not do

The calculator performs mathematical conversions only. It does not recommend, suggest or verify any Thymosin Alpha-1 dose or schedule, it does not say how much water to add, and it does not say what Thymosin Alpha-1 is for, whether it is safe, or whether it is legal where you live. It is not medical advice, diagnosis or treatment, and Peptrack does not sell, supply or endorse any substance.

Always confirm measurements with a licensed healthcare provider or pharmacist. See the full disclaimer.

Zadaxin is a trademark of its owner. Peptrack is not affiliated with, or endorsed by, any manufacturer.

Sources

Facts last checked: September 2026. Regulatory status changes, so check the regulator’s own pages for the current position.

Thymosin Alpha-1 Calculator FAQ

How do I calculate thymosin alpha-1 concentration after reconstitution?

Divide the milligrams in the vial by the millilitres of liquid added. A 1.6 mg vial with 1 mL is 1.6 mg/mL, so 10 units on a U-100 syringe holds 0.16 mg. A 5 mg vial with 2 mL is 2.5 mg/mL, so 10 units holds 0.25 mg. These figures illustrate the arithmetic and are not a suggestion of what to use.

Is thymosin alpha-1 the same as TB-500?

No. They are different molecules. Thymosin alpha-1 has 28 amino acids. TB-500 is a fragment of thymosin beta-4, which has 43. They share a name only because both were first identified in thymus extracts.

What is the half-life of thymosin alpha-1?

Published reviews give a half-life in the circulation of about 2 hours. Half-life is a property of the compound and plays no part in the calculator’s arithmetic.

Is thymosin alpha-1 approved, and does this calculator recommend a dose?

Thymalfasin, the synthetic form, is approved as a prescription medicine in more than 35 countries but has never been approved by the FDA. This calculator never recommends, suggests or checks a dose. It converts the numbers you enter, for research and informational reference only, and it is not medical advice.

The same math, with each compound’s own vial sizes and units.

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