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Free Peptide Calculator

Peptide reconstitution, draw volume & syringe units

A free peptide reconstitution and dose calculator. Type in your vial size, the bacteriostatic water you added and the dose you want to convert, and it returns the concentration, the draw volume in mL and the exact syringe units on a U-100 insulin syringe. It does the arithmetic. It never suggests a dose.

Enter your numbers

mg

Total amount in the vial, as printed on its label.

mL

How much water went into the vial.

Dose unit

Enter the dose from your own records or prescription. This tool never suggests one.

Syringe size (U-100 insulin syringe)

Draw volume on a 1 mL U-100 syringe

Fill in the three fields and the math appears here instantly.

102030405060708090100
Concentration
Per unit mark
per U-100 unit
Draws per vial
at the dose entered

Arithmetic on the numbers you entered. Not a dose recommendation and not medical advice.

Tired of redoing this? The free Peptrack app saves the concentration with each vial and keeps your log and reminders in one place.

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How the peptide calculator works

Lyophilized (freeze-dried) peptides come as a powder measured in milligrams. Once a known volume of bacteriostatic water is added, the vial holds a solution with a specific concentration. From that point on, every amount of peptide corresponds to a volume of liquid, and every volume corresponds to a mark on a syringe. This peptide calculator converts between those three things.

  1. Enter the vial size in mg. This is the total amount printed on the vial label.
  2. Enter the BAC water you added in mL. The calculator divides the two to get the concentration.
  3. Enter the dose to convert, in mcg or mg. This comes from your own records or prescription. The calculator converts it to a draw volume and shows where that falls on a U-100 syringe.

Everything runs in your browser. Nothing you type is uploaded, there is no signup, and results update as you type.

The peptide reconstitution formula

There is no hidden logic. The whole tool is three lines of arithmetic, and the “Show the math” panel prints them with your own numbers.

1. Concentration

Concentration (mg/mL) = vial size (mg) ÷ water added (mL)

2. Draw volume

Volume (mL) = amount (mg) ÷ concentration (mg/mL)

If the amount is in micrograms, it is divided by 1,000 first to convert it to milligrams.

3. Syringe units

U-100 units = volume (mL) × 100

A U-100 insulin syringe has 100 unit marks per millilitre, so one unit is 0.01 mL.

Worked example of the math

The numbers below are arbitrary and chosen only because they divide cleanly. They illustrate the formula and are not a suggested amount for anything.

  • Vial size: 5 mg. Water added: 2 mL.
  • Concentration: 5 ÷ 2 = 2.5 mg/mL (2,500 mcg/mL, or 25 mcg per unit mark).
  • Amount entered: 250 mcg, which is 250 ÷ 1,000 = 0.25 mg.
  • Volume: 0.25 ÷ 2.5 = 0.1 mL.
  • Syringe units: 0.1 × 100 = 10 units on a U-100 syringe.

Press “Try example numbers” in the calculator above to see the same values on the syringe illustration.

Insulin syringe types: U-100, U-50 and U-40

The “U” number describes how a syringe’s scale is printed. Reading a result against the wrong scale changes the volume, so it is worth knowing which one is in your hand.

Comparison of insulin syringe scales
SyringeMarks per mL1 unit equalsTypical barrels
U-100100 units0.01 mL0.3 mL (30 units), 0.5 mL (50 units), 1 mL (100 units)
“U-50”100 units0.01 mLCommon shorthand for the 0.5 mL, 50-unit U-100 syringe
U-4040 units0.025 mL1 mL (40 units), mostly veterinary

U-100 is the standard insulin syringe and the scale this calculator displays. The 0.3 mL, 0.5 mL and 1 mL versions all use the same unit size. A smaller barrel simply spreads the marks further apart, which makes small volumes easier to read.

“U-50” is usually shorthand for a 0.5 mL U-100 syringe that holds 50 units. Its unit marks mean exactly the same volume as on any other U-100 syringe, so you can select “0.5 mL” in the calculator.

U-40 syringes are genuinely different. They have only 40 marks per mL, so one U-40 unit is 0.025 mL, which is 2.5 times the volume of a U-100 unit. To express a volume on a U-40 scale, multiply the mL figure by 40 instead of 100. Always check the scale printed on the barrel.

Unit conversions: mcg, mg and mL

  • 1 mg = 1,000 mcg. 0.5 mg is 500 mcg, and 250 mcg is 0.25 mg.
  • 1 mL = 100 units on a U-100 syringe. 0.1 mL is 10 units, and 0.05 mL is 5 units.
  • mg and mL are not interchangeable. Milligrams measure an amount of powder. Millilitres measure a volume of liquid. The concentration is what links them.
  • Syringe units are not IU. The International Units on some product labels measure biological activity and are specific to each substance. They cannot be converted here.

Common arithmetic slips this helps you catch

  • mcg entered as mg. This shifts the result by 1,000×. The calculator flags any amount larger than the whole vial.
  • Units read as mL. 10 units is 0.1 mL, not 10 mL. Both figures are shown side by side.
  • The wrong syringe scale. A result in U-100 units does not apply to a U-40 barrel.
  • Reusing an old concentration. A new vial size or a different water volume means a new concentration. Recalculate for each vial.
  • A volume too large for the barrel. The calculator tells you when a result exceeds the syringe size you selected.

Want the concentration stored with each vial so you never retype it? The free Peptrack app saves it alongside your vial inventory and dose log.

What this calculator does not do

This is a math tool, and it is important to be clear about where it stops.

  • It does not recommend, suggest or verify any dose, schedule or protocol.
  • It does not know what is in your vial, and it has no information about you.
  • It does not decide how much water to add. If the instructions from your pharmacy, manufacturer or healthcare provider give a volume, use that one. To see how different volumes would read on a syringe, compare them in the BAC water calculator.
  • It does not provide medical advice, diagnosis or treatment.

Results are for research, informational and record-keeping reference only. Always confirm measurements with a licensed healthcare provider or pharmacist. See the full disclaimer.

Looking for a different angle on the same math? Try the reconstitution calculator, the BAC water calculator or the peptide dosage calculator, or head back to the Peptrack homepage.

Peptide Calculator FAQ

What does the peptide calculator do?

It does three pieces of arithmetic on numbers you enter. It divides the vial size by the water added to get a concentration in mg/mL, divides the amount you typed by that concentration to get a volume in mL, and multiplies the volume by 100 to show the matching mark on a U-100 insulin syringe. It does not suggest any amount.

How do you calculate peptide concentration after reconstitution?

Concentration equals the amount of powder in the vial divided by the volume of liquid added. For example, 5 mg in 2 mL is 5 ÷ 2 = 2.5 mg/mL, which is the same as 2,500 mcg/mL or 25 mcg per unit on a U-100 syringe.

How do I convert mcg to units on an insulin syringe?

First convert mcg to mg by dividing by 1,000. Divide that by the concentration in mg/mL to get mL, then multiply by 100 to get U-100 units. With a 2.5 mg/mL concentration, 250 mcg is 0.25 mg, 0.25 ÷ 2.5 = 0.1 mL, and 0.1 × 100 = 10 units. These are example numbers that illustrate the formula, not a suggested amount.

What does “units” mean on a U-100 syringe?

On an insulin syringe, units are a measure of volume. A U-100 syringe has 100 unit marks per millilitre, so 1 unit is 0.01 mL and 10 units is 0.1 mL. This is different from the International Units (IU) of biological activity printed on some product labels, which cannot be converted with this calculator.

Does adding more BAC water change how much is in the vial?

No. The total amount in the vial stays the same. More water only lowers the concentration, which means a larger volume holds the same amount. If the instructions from your pharmacy, manufacturer or healthcare provider give a water volume, use that one and enter it here. To compare how different volumes would read on a syringe, use the BAC water calculator.

Does this calculator tell me what dose to take?

No. It has no information about any compound, person or condition, and it never recommends, suggests or checks an amount. It only converts the values you enter. What to take and how much are decisions for you and a licensed healthcare provider.

What is the difference between mcg and mg?

A milligram (mg) is 1,000 micrograms (mcg). 250 mcg is 0.25 mg, and 2 mg is 2,000 mcg. Mixing the two up changes a result by a factor of 1,000, so the calculator has an mcg/mg switch and shows the converted value in its working.

Can I use this for GLP-1 vials?

The arithmetic is the same for any powder reconstituted with a known volume of liquid, and the calculator never asks what is in the vial. Pre-filled pens are already measured by the manufacturer and do not need this math. Always follow the instructions from your pharmacy and prescriber.

Is the peptide calculator free? Do I need to sign up?

It is completely free, with no signup and no login. The math runs in your browser, so it works instantly and the numbers you type never leave your device.

Same math engine, framed for a different question. All free, all instant, no signup.

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Peptrack saves each vial's concentration, logs every dose you record and reminds you on the schedule you set. 100% free, with no ads.

Free on iOS & Android. A record-keeping tool, not medical advice.